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About The Book
This one has been written to meet the requirements of the Bachelor's course in pharmacy as per the syllabi prescribed by All India Council for Technical Education and adopted by other universities including the Universities of Health Sciences. The theoretical part has been divided into 18 hapters. Due emphasis on cell structure, transport mechanism, metabolic pathways, molecular biology, biochemical functions of hormones have been given. A few questions have been given at the end of each chapter to help the students in preparing for the examinations.
Some of the chapters i.e. Biological oxidation, Enzymes, Carbohydrate metabolism, Biosynthesis of nucleic acids have been updated. In the Practical part, qualitative tests for carbohydrates, proteins, lipids and a few exercises as per syllabi prescribed by All India Council for Technical Education have been included.
The book also includes the Practical Exercises within the limits of approved syllabi in pharmaceutical biochemistry along with the interpretations.
The book will be found interesting by the students and faculty of pharmacy and also of medical, dental and other allied fields.
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PART I — Theory
1. The Cell and Transport through Membranes
2. Bioenergetics
3. Biologic Oxidation
4. Enzymes
5. Metalloenzymes, Coenzymes and Vitamins
6. Carbohydrate Metabolism
7. Lipid Metabolism
8. The Nitrogen and Sulphur Cycles
9. Metabolism of NH3 and Nitrogen containing Monomers
10. Biosynthesis of Nucleic Acids
11. Protein Biosynthesis
12. Regulation of Gene Expression
13. Organ Function Tests
14. Hormones
PART II — Practicals
1. Buffers and pH
1-1 Preparation of standard buffer solutions
1-2 Measurement of pH
2. Titration Curve for Amino Acids
3. Special Techniques for Separation of Mixtures
3-1 Electrophoresis
3-2 Chromatography
3-3 Electrophoresis of Serum Proteins
3-4 Separation of Amino Acids by two dimensional paper chromatography
3-5 Two dimentional thin layer chromatography for Urinary Amino Acids
3-6 Two dimensional separation of Amino Acids by electrophoresis followed
by chromatography
3-7 Separation of Lipids by thin layer chromatography
4. (1) Identification of C-Terminal Amino Acid of a Protein
(2) Identification of N-Terminals Amino Acid of a Protein
5. Isolation and Assay of Glycogen from Liver and Skeletal Muscle of Rats
6. Enzymic Hydrolysis of Glycogen by α and β Amylase
7. Effect of Temperature & pH on the Activity of β Amylase
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8. Isolation and Determination of RNA and DNA
8-1 Isolation of RNA
8-2 Estimation of RNA by Orcinol reaction
8-3 Isolation of DNA from pig spleen
8-4 Estimation of DNA by diphenylamine reaction
9. Qualitative Tests for Carbohydrates, Proteins and Lipids
Tests for Carbohydrates; Proteins ;
Lipids ; Glycerol ; Cholesterol
10. Determination of Blood Constituents
Colorimetry
10-1(a) Blood glucose
10-1(b) Carbohydrates by Anthrone Method
10-1(c) Reducing sugars using DNS
10-2 Serum proteins
10-3 SGOT and SGPT
10-4 Serum bilirubin
10-5 Serum alkaline phosphatase
10-6 Serum cholesterol
10-7 Serum calcium
10-8 Urine calcium
10-9 Serum urea
10-10 Serum creatinine
10-11 Urine creatinine
10-12 Serum and urine chlorides
10-13 Urinary Acidity and Ammonia
11. Flame Photometry—Determination of Sodium and Potassium in Serum
and Urine.
12. Milk
12-1 Lactose ; 12-2 Casein
13. Normal Urine
14. Abnormal Constituents of Urine
15. Quantitative Estimation of Reducing Sugar in Urine
16. Liver Function Tests
17. Renal Function Tests
18. Gastric Juice
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