About The Book
The contents are suitable to pharma students of D. Pharm, B. Pharm, M. Pharm and research scholars and follows PCI syllabus. The book comprises many chapters such as Extraction and isolation of herbals, herbal formulations, pharmacological screening, etc.
The macroscopic as well as the microscopic description will help to remember the salient features regarding the identification of crude drugs. The phytochemical part covers the tests for various classes of plant constituents and also the experimental procedures describing ex¬traction and isolation.
Simple language, point-wise information, reader-friendly presentation, well-labelled diagrams and coverage of all important aspects of practicals constitute the special features of this book.
Content
1.Introduction to Laboratory Instruments
A. Soxhlet Apparatus
B. Hot Air Oven
C. Lyophilizer
D. Muffle Furnace/Retort Furnace
E. Colony Counter
F. Antibiotic Zone Reader
G. Desiccator
H. BOD Incubator
2.Microscope and its Techniques
A. Microscope
B. Microtome
C. Section Cutting Technique
D. Camera Lucida
3.The Cell and Tissue System
A. Plant Cell
B. Plant Tissue
4.Introduction to Different Parts of Plants
A. Leaf (Phyllopodium)
B. Flowers
C. Fruits/Pericarp
D. Seeds
E. Stems
F. Bark/Periderm
G. Woods
H. Roots
5.Microscopical Studies of Crude Drugs
A. Fennel Fruit
B. Coriander Fruit
C. Nux-vomica Seed
D. Isapgol Seed
E. Senna Leaf
F. Datura Leaf
G. Neem Leaf
H. Vasaka Leaf
I. Vinca Leaf
J. Clove Bud
K. Ginger Rhizome
L. Ipecac Root
M. Shatavari Root
N. Ephedra Stem
O. Cinchona Bark
P. Kurchi Bark
Q. Liquorice Root
R. Cinnamon Bark
6.Extraction Techniques of Medicinal Plants
A. Methods of Extraction of Medicinal Plants
B. Methods of Isolation of Volatile Oils
C. Solvents Used in Extraction Processes
7.Extraction And Isolation of Phytoconstituents
A. Isolation of Gingerol and Shagaol from Ginger
B. Isolation of Andrographolide from Kalmegh
C. Isolation of Capsaicin from Capsicum
D. Isolation of Atropine from Datura
E. Isolation of Diosgenin from Dioscorea
F. Isolation of Glycyrrhetinic acid from Liquorice
G. Isolation of Guggulsterone from Guggul
H. Isolation of Nicotine from Tobacco
I. Isolation of Piperine from Black Pepper
J. Isolation of Sennosides from Senna
K. Isolation of Strychnine and Brucine from Nux Vomica
L. Isolation of Curcumin from Turmeric
M. Isolation of Eugenol from Clove
N. Isolation of Vasicine from Vasaka
O. Isolation of Vinblastine and Vincristine from Vinca
P. Isolation of Caffeine from Tea
Q. Isolation of Digoxin from Digitalis
R. Isolation of Hesperidin from Orange Peel
8.Chromatographic Techniques
A. Thin Layer Chromatography (TLC)
B. Paper Chromatography
C. Column Chromatography
D. High Performance Liquid Chromatography (HPLC)
E. High Performance Thin Layer Chromatography (HPTLC)
F. Gas Chromatography (GC)
9.Evaluation Techniques
A. Organoleptic Evaluation/Morphological Evaluation/Sensory Evaluation
B. Microscopical Evaluation
I. Stomatal number
II. Stomatal index
III. Palisade ratio
IV. Vein islet number
V. Vein termination number
VI. Lycopodium spore method
C. Physical Evaluation
I. Determination of melting point
II. Determination of boiling point
III. Determination of optical activity
IV. Determination of refractive index
V. Determination of total ash value
VI. Determination of acid insoluble ash value
VII. Determination of water soluble ash value
VIII.Determination of sulphated ash value
IX. Deter. of water-soluble extractive value
X. Deter. of alcohol soluble extractive values
XI. Determination of moisture content
XII. Determination of volatile oil content
XIII. Determination of crude fiber
D. Chemical Evaluation
I. Preliminary phytochemial screening
E. Biological Evaluation
I. Determination of bitterness value
II. Determination of haemolytic index
III. Determination of tannin content
IV. Determination of swelling index
V. Determination of foaming index
VI. Determination of aflatoxins
VII. Determination of microbial contamination
VIII. Determination of heavy metals
10.Plant Tissue Culture Techniques and Experiments
A. Some Experiments
B. Preparation of Media MS Medium
C. Surface Sterilization of Explants
D. Staining Techniques
E. Gram Staining
F. Acid-Fast Staining
11.Herbal Formulations
A. Herbal Cream
B. Herbal Shampoo
C. Herbal Syrup
D. Herbal Lipstick
E. Asava and Arishta
F. Vati or Gutika
G. Taila
H. Churnas
I. Avaleha or Lehya
J. Bhasmas
K. Arka
12.Monograph Analysis of Herbal Drugs as per WHO
A. Folium Sennae
B. Aloe
C. Radix Glycyrrhizae
D. Radix Rauwolfiae
E. Rhizoma Zingiberis
13.Evaluation of Herbal Excipients
A. Evaluation of Some Frequently Used Herbal Excipients
14.Pharmacological Screening of Herbals
A. Anti-inflammatory Activity
B. Antipyretic Activity
C. Antiulcer Activity
D. Antidiabetic Activity
E. Analgesic Activity
F. Hepatoprotective Activity
G. Diuretic Activity
H. Antioxidant Activity
I. Immunomodulatory Activity
J. Aphrodiasiac Activity
15.Some Important Experiments
A. Experiments 1 to 12
Reagents and Chemicals
Subject Index
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